platelet derived growth factor receptor beta pdgfrβ  (Boster Bio)

Journal: International Journal of Molecular Sciences

Article Title: In Vivo Target Engagement Assessment of Nintedanib in a Double-Hit Bleomycin Lung Fibrosis Rat Model

doi: 10.3390/ijms27010064

Figure Lengend Snippet: Assessment of Nintedanib target engagement. Nintedanib (light blue columns) did not modify lung levels of PDGF ( A ), which was unaffected by BLM, while significantly attenuated FGF2 lung levels ( B ) were enhanced by BLM. In contrast, lung ( C ) and plasma ( D ) VEGF levels were significantly increased by Nintedanib treatment relative to both untreated (control) and BLM treated groups. Panel ( E , F ) illustrates the rise in VEGF levels in lung and plasma, respectively, observed at different time points following Nintedanib treatment in BLM-free animals. Statistical analysis was performed with the one-way ANOVA followed by Dunnett’s test. A p -value < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01 when compared to the control group (cream columns, Panels A – D ) or time points 0 (Panels E , F ). # p < 0.05 when compared to BLM + Vehicle group (teal blue columns, Panels A – D ).

Article Snippet: All markers were quantified by Enzyme-linked immunosorbent assays (ELISA) commercial kits: procollagen-I (ab210579, Abcam, Cambridge, UK), WISP-1 (Mouse/Rat WISP-1/CCN4 Quantikine ELISA Kit, MWSP10, Bio-Techne, Minneapolis, MN, USA), MMP7 (Rat MMP-7 ELISA Kit, NBP3-06896, Bio-Techne, Minneapolis, MN, USA), PDGF (Rat PDGF ELISA Kit, orb567597, Biorbyt Cambridge, UK), FGF2 (Mouse and Rat FGF basic/FGF2/bFGF ELISA Kit—Quantikine, MFB00, Bio-Techne, Minneapolis, MN, USA) and VEGF-A (Rat VEGF-A ELISA Kit, ab100787, Abcam, Cambridge, UK).

Techniques: Drug discovery, Clinical Proteomics, Control, Cream

Journal: International Journal of Molecular Sciences

Article Title: In Vivo Target Engagement Assessment of Nintedanib in a Double-Hit Bleomycin Lung Fibrosis Rat Model

doi: 10.3390/ijms27010064

Figure Lengend Snippet: Down-regulated pathways derived from phosphorylated proteins. The down-regulated proteins enrich pathways associated with VEGF, PDGF, and FGF, mainly 3 h after the administration. The blue shades indicate the q-value.

Article Snippet: All markers were quantified by Enzyme-linked immunosorbent assays (ELISA) commercial kits: procollagen-I (ab210579, Abcam, Cambridge, UK), WISP-1 (Mouse/Rat WISP-1/CCN4 Quantikine ELISA Kit, MWSP10, Bio-Techne, Minneapolis, MN, USA), MMP7 (Rat MMP-7 ELISA Kit, NBP3-06896, Bio-Techne, Minneapolis, MN, USA), PDGF (Rat PDGF ELISA Kit, orb567597, Biorbyt Cambridge, UK), FGF2 (Mouse and Rat FGF basic/FGF2/bFGF ELISA Kit—Quantikine, MFB00, Bio-Techne, Minneapolis, MN, USA) and VEGF-A (Rat VEGF-A ELISA Kit, ab100787, Abcam, Cambridge, UK).

Techniques: Derivative Assay

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A The images of HK-2 cells treated with different concentrations of H. pluvialis were captured by an inverted optical microscope ( n = 3). B CCK-8 viability assay evaluating HK-2 cell proliferation under various doses and treatment durations of H. pluvialis ( n = 3). C ELISA quantification of CTGF and PDGF secretion in HK-2 cells ( n = 3). D qRT-PCR analysis of SASP markers in HK-2 cells ( n = 3). ACTB was used as a reference gene. E GSEA signaling enrichment analyses in TGF-β1-stimulated HK-2 cells via RNA-seq. F Functional enrichment analysis of CCN2 gene and SERPINE1 gene via RNA-seq profiling. * p < 0.05 vs. Con group, # p < 0.05 vs. TGF-β1 group. For GSEA interpretation, thresholds were set at |normalized enrichment score (NES)| >1, nominal p < 0.05, and FDR q < 0.5.

Article Snippet: The levels of connective tissue growth factor (CTGF) and platelet-derived growth factor (PDGF) were determined using Human CTGF ELISA Kit (EK198, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China) and Human PDGF ELISA Kit (EK12318, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China).

Techniques: Microscopy, CCK-8 Assay, Viability Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, RNA Sequencing, Functional Assay

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A Blue: Nuclei. Purple: Mitochondrial. Red: PINK1/Parkin/p62/LC3B. IF staining was performed to detect PINK1, Parkin, p62, and LC3B protein levels (magnification: 100×, scale bar: 15 μm). B Colocalization analysis of p62, LC3B, and mitochondria was performed using ImageJ software. C Colocalization Pearson correlation coefficient analysis were obtained based on IF images of p62 and LC3B ( n = 6). D Western blot analysis of PINK1 and LC3B expression in renal cortical tissues from different experimental groups. Quantification of gray values for Western blotting results in ( D ) was performed using ImageJ ( n = 3). E Blue: Nuclei. Red: E-cadherin/N-cadherin/Vimentin. IF assay was performed to detect E-cadherin, N-cadherin and Vimentin protein levels (magnification: 100×, scale bar: 15 μm). F Quantitative analysis of fluorescence intensity using ImageJ software ( n = 6). G ELISA quantification of CTGF and PDGF secretion in HK-2 cells ( n = 3). H Western blot analysis of E-cadherin, N-cadherin and Vimentin expression in TGF-β1-induced HK-2 cells. Quantification of gray values for Western blotting results in ( H ) was performed using ImageJ ( n = 3). I Representative flow cytometry plots showing the detection of MMP. J Quantitative analysis of flow cytometry fluorescence intensity of ROS ( n = 3). * p < 0.05 vs. Con group, # p < 0.05 vs. TGF-β1 group. a p < 0.05 vs. HP group, b p < 0.05 vs. AST group.

Article Snippet: The levels of connective tissue growth factor (CTGF) and platelet-derived growth factor (PDGF) were determined using Human CTGF ELISA Kit (EK198, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China) and Human PDGF ELISA Kit (EK12318, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China).

Techniques: Staining, Software, Western Blot, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Flow Cytometry